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Lumex Instruments (0890278 B.C. Ltd.) Unit 207, 31510 Gill Ave., Mission, B.C., V4S0A1, Canada 31510 (0890278 B.C. Ltd.) Unit 207, 31510 Gill Ave., Mission, B.C., V4S0A1, Canada
+1 866-233-6057, +1 604 820-4486, sales@lumexinstruments.com

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Capillary electrophoresis system Capel-205

Capillary electrophoresis system Capel-205

Capel-205 is a new generation capillary electrophoresis system with a significantly extended autosampler for continuous running of dozens of analyses and increased efficiency in separation.
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Capel-205 is a new generation capillary electrophoresis system with advanced options and design. Its main features are:

  • Extended autosampler capacity up to 59 positions, allowing to provide overnight analyses
  • Standard centrifuge-type vials of 1.5mL volume (Eppendorf-type) closed with their original caps are used for all solutions
  • Specially designed unit automatically opens vials just prior to their use, thus preventing evaporation and any kinds of cross-contamination
  • New HV block provides voltage up to +/- 30 kV thus improving separation efficiency and decreasing analysis time
  • Optionally – integrated unit to control the state of the inner capillary surface provides significantly improved migration time reproducibility especially when analyzing samples with complex matrices

Thus Capel-205 can be the very best choice not only for R&D and low-scale analyses but also for the customers dealing routinely with dozens of samples per day.

principle of operation

High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.

features and benefits
Extended instrumental options
  • Extended autosampler capacity up to 59 positions – hundreds of samples per months can be analyzed
  • Integrated vials caps removal – all solutions are kept in the autosampler tightly closed excluding evaporation and cross-contamination
  • New HV block provides voltage up to +/- 30 kV – separation efficiency is improved and analysis time is decreased
  • Redesigned capillary cassette – length of the thermostated part of the capillary is increased to improve migration time reproducibility
  • Spectra scanning facilitates peak identification
  • Broad range of controlled injection pressure allows analysis of viscous samples
  • Reverse sample injection under vacuum: ultra-short analysis time (less than 1 min) and sample stacking to decrease detection limit
Precise and highly effective capillary thermostating provided by circulating liquid coolant (water)(± 0.1 C)
  • Extended range of applied buffers and increased efficiency in separation
Powerful software package "Elforun"
  • Modified Elforun-205 to become even more user-friendly when operating with extended autosampler capacity
  • Increased flexibility in performing analyses of various complexity
  • Any kinds of complex runs are possible including those with pre-programming of changes in analysis conditions
  • Customized report, data export to other programs
Unique design of the capillary cassettes
  • Enlarged thermostatic part of the capillary improves migration time reproducibility
  • Easy capillary change in just a few minutes
  • Lower Detection Limit due to the optimized optical scheme
Analytical characteristics

Detectors
CAPEL-205 is equipped with a spectrophotometric detector with a wavelength range of 190 - 400 nm.

Light source
Deuterium lamp

Vials
Standard centrifuge-type (e.g.Eppendorf-type)1.5 ml, minimal sample volume 100 µl.

Injection modes
Sample injections by pressure, vacuum or electrokinetically.
Controlled pressure profile of injection with pressure values from – 100 mbar to + 100 mbar.
Electrokinetic injection at (+/-) 1 – 30 kV considering time for voltage ramping.
Programmable injection time / pressure / voltage.

Analysis
With a Voltage from -30 kV to +30 kV with a 1 kV step and an electronic polarity switch.
Current from 0 to 300 µA.

Elforun software features

  • Combination of instrument controlling, data acquisition and data processing;
  • Real time electropherogram visualization;
  • Electropherogram data processing with overlay mode and basic mathematical operations;
  • Computation of electrophoresis system parameters;
  • Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors;
  • Extensive data processing algorithm customization;
  • GLP and GALP compliance.
equipment and options
  • Capillary electrophoresis system CAPEL-205;
  • Spare capillary cassettes;
  • Elforun software package;
  • Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel-205.

Warranty

All Lumex instruments and equipment are covered by a 12-months warranty.

services

Installation of LUMEX instruments can be carried out at a Customer's site by our service engineers. Personnel training specific to the Customer needs can be also provided.

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Detector Spectrophotometer
190-400 nm
Analysis Constant voltage, from –30 kV to +30 kV in 1 kV steps
Automatic (electronic) polarity switch
Current 0 - 300 mkA
Pressure up to 100 mbar
Programmable changing of wavelength, pressure and voltage during analysis
Injection By voltage, from –30 kV to +30 kV in 1 kV steps;
By pressure, from –100 to 100 mbar in 1 mbar steps
Sampler Autosamplers for 59 vials (standard Eppendorf-type 1.5 mL)
Rinsing By pressure, 500–2000 mbar in 1 mbar steps
Capillary Length: 30–120 cm
Internal diameter: 50, 75 μm
Capillary thermostating Liquid thermostating, from -10 up to+ 30 °C from ambient temperature, ±0.1°C
Controlled parameters Wavelength
Pressure
Temperature
Voltage
Power requirements 110–240 Vac, 50/60 Hz
Power consumption 170 W
Size, LxWxH, mm 470х530х410
Weight, kg 30
areas of application
  • Environmental analysis: water and soil quality monitoring (cations, anions, herbicides, pesticides);
  • Food industry: analysis of beverages and juices (cations, anions, vitamins, organic acids, preservatives, antioxidants, dyes, sweeteners); mineral water (cations, anions); wines and brandies (cations, organic acids, polyphenols, aldehydes, sulfite); beer (inorganic cations and anions, hop and bitter acids, sulfite, amino acids, organic acids, vitamins); milk (proteins, antibiotics); tea, coffee (caffeine, polyphenols); foodstuff (amino acids, synthetic dyes, organic acids, amines, proteins, melamine);
  • Agriculture: grain and grain products (amino acids), mixed fodders (cations, amino acids, amines, vitamins);
  • Chemical industry: technological monitoring, analysis of raw material;
  • Pharmaceutical industry: patent medicines analysis, technological monitoring, and enantiomers separation;
  • Biochemistry: analysis of proteins by capillary isoelectric focusing technique, pharmacokinetics studies, hemoglobin variants determination;
  • Forensic studies: analysis of explosives and trace detection, drugs analysis, analysis of writing paper components.
Application Product
Determination of proteinogenous amino acids in feedstuffs, compound feeds, and feed raw materials Determination of proteinogenous amino acids in feedstuffs, compound feeds, and feed raw materials

Capel-105M

Capel-205

Determination of lysine and its salts in fodder additives Determination of lysine and its salts in fodder additives

Capel-105M

Capel-205

Determination of choline (vitamin B4) in feedstuffs, compound feeds, fodder additives, and feed raw materials Determination of choline (vitamin B4) in feedstuffs, compound feeds, fodder additives, and feed raw materials

Capel-105M

Capel-205

Determination of cations in feedstuffs, compound feeds, and feed raw materials Determination of cations in feedstuffs, compound feeds, and feed raw materials

Capel-105M

Capel-205

Determination of methionine hydroxy analogue in fodder additives Determination of methionine hydroxy analogue in fodder additives

Capel-105M

Capel-205

Determination of 4-carboxybenzaldehyde & p-toluic acid in purified terephthalic acid according to ASTM D7881 & ASTM D7882 test methods Determination of 4-carboxybenzaldehyde & p-toluic acid in purified terephthalic acid according to ASTM D7881 & ASTM D7882 test methods

Capel-105M

Capel-205

Determination of synthetic amino acids (methionine, threonine, tryptophan) in fodder additives Determination of synthetic amino acids (methionine, threonine, tryptophan) in fodder additives

Capel-105M

Capel-205

Determination of organic acids in feedstuffs and fodder additives Determination of organic acids in feedstuffs and fodder additives

Capel-105M

Capel-205

Determination of ascorbic acid and its salts in food additives and fodder additives Determination of ascorbic acid and its salts in food additives and fodder additives

Capel-105M

Capel-205

Determination of anions in beverage Determination of anions in beverage

Capel-105M

Capel-205

Determination of anions in feedstuffs, compound feeds, and feed raw materials Determination of anions in feedstuffs, compound feeds, and feed raw materials

Capel-105M

Capel-205

Determination of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements Determination of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements

Capel-105M

Capel-205

Determination of preservatives (benzoic, sorbic acids, and their salts) and sweeteners (acesulfame K, saccharine, and its salts) in food products and raw materials, and dietary supplements Determination of preservatives (benzoic, sorbic acids, and their salts) and sweeteners (acesulfame K, saccharine, and its salts) in food products and raw materials, and dietary supplements

Capel-105M

Capel-205

Determination of acetic acid (acetate anions) in natural, drinking, and waste water Determination of acetic acid (acetate anions) in natural, drinking, and waste water

Capel-105M

Capel-205

Determination of caffeine, ascorbic acid, preservatives (benzoic acid, sorbic acid and their salts), and artificial sweeteners (acesulfame K, saccharine) in soft and strong drinks Determination of caffeine, ascorbic acid, preservatives (benzoic acid, sorbic acid and their salts), and artificial sweeteners (acesulfame K, saccharine) in soft and strong drinks

Capel-105M

Capel-205

Determination of water-soluble forms of inorganic cations in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments Determination of water-soluble forms of inorganic cations in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments

Capel-105M

Capel-205

Determination of bromide and iodide anions in water samples Determination of bromide and iodide anions in water samples

Capel-105M

Capel-205

Determination of chlorate, perchlorate, and chlorite ions in drinking water including bottled water Determination of chlorate, perchlorate, and chlorite ions in drinking water including bottled water

Capel-105M

Capel-205

Determination of water-soluble forms of inorganic and organic anions in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments Determination of water-soluble forms of inorganic and organic anions in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments

Capel-105M

Capel-205

Determination of inorganic anions in aqueous matrices according to EPA 6500 and ASTM D6508-10 test methods Determination of inorganic anions in aqueous matrices according to EPA 6500 and ASTM D6508-10 test methods

Capel-105M

Capel-205

Determination of inorganic cations in water samples Determination of inorganic cations in water samples

Capel-105M

Capel-205

Determination of inorganic anions in water samples Determination of inorganic anions in water samples

Capel-105M

Capel-205

Determination of fructose, glucose, and saccharose in beverages, fruit and vegetable products, honey, and bioactive food additives Determination of fructose, glucose, and saccharose in beverages, fruit and vegetable products, honey, and bioactive food additives

Capel-105M

Capel-205

Determination of serum protein fractions Determination of serum protein fractions

Capel-105M

Capel-205

Determination of protein purity and heterogeneity with capillary gel electrophoresis and capillary isoelectric focusing Determination of protein purity and heterogeneity with capillary gel electrophoresis and capillary isoelectric focusing

Capel-105M

Capel-205

Identification and determination of synthetic dyes in soft and alcoholic drinks Identification and determination of synthetic dyes in soft and alcoholic drinks

Capel-105M

Capel-205

Determination of vanillin, sinapic, coniferyl, and syringic aldehydes in brandies and brandy spirits Determination of vanillin, sinapic, coniferyl, and syringic aldehydes in brandies and brandy spirits

Capel-105M

Capel-205

Determination of proteins concentrations and their molecular weights Determination of proteins concentrations and their molecular weights

Capel-105M

Capel-205

Determination of bitter iso- alpha-acids and alpha- and beta-hop acids in beer Determination of bitter iso- alpha-acids and alpha- and beta-hop acids in beer

Capel-105M

Capel-205

Determnation of albumin concentration in urine Determnation of albumin concentration in urine

Capel-105M

Capel-205

Determination of phenoxycarboxylic acid herbicides in water Determination of phenoxycarboxylic acid herbicides in water

Capel-105M

Capel-205

Determination of organic acids in beverages Determination of organic acids in beverages

Capel-105M

Capel-205

Determination of inorganic cations in drinks Determination of inorganic cations in drinks

Capel-105M

Capel-205

Determination of D- and L-isomers of tartaric and malic acids in wines, wine materials, and food additives Determination of D- and L-isomers of tartaric and malic acids in wines, wine materials, and food additives

Capel-105M

Capel-205

Determination of organic acids in feedstuff, silage, haylage, and fermentates for biogas plants Determination of organic acids in feedstuff, silage, haylage, and fermentates for biogas plants

Capel-105M

Capel-205

Determination of organic acids and some preservatives in wine and spirits Determination of organic acids and some preservatives in wine and spirits

Capel-105M

Capel-205

Determination of total sulfur dioxide in wine and beer Determination of total sulfur dioxide in wine and beer

Capel-105M

Capel-205

Determination of hesperidin and naringin in juices Determination of hesperidin and naringin in juices

Capel-105M

Capel-205

Determination of the carbendazim content in orange and other citrus juice produce Determination of the carbendazim content in orange and other citrus juice produce

Capel-105M

Capel-205

Determination of whey proteins in milk samples by capillary gel electrophoresis Determination of whey proteins in milk samples by capillary gel electrophoresis

Capel-105M

Capel-205

Determination of amino acids in beer and wort Determination of amino acids in beer and wort

Capel-105M

Capel-205

Webinars

CE webinar: Fast, Accurate, and Reliable Chemical Analysis of Water


CE Webinar: How to get insight into wines and spirits


Analysis of recombinant protein purity and heterogeneity by cappliary electrophoresis