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Lumex Instruments (0890278 B.C. Ltd.) Unit 207, 31510 Gill Ave., Mission, B.C., V4S0A1, Canada 31510 (0890278 B.C. Ltd.) Unit 207, 31510 Gill Ave., Mission, B.C., V4S0A1, Canada
+1 866-233-6057, +1 604 820-4486, sales@lumexinstruments.com

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Analysis of recombinant protein purity and heterogeneity by capillary electrophoresis
CE System Capel 105M

CE System Capel 105M

Capel-105M is a high performance capillary electrophoresis system for fast and effective solutions in food industry, environmental applications, pharmaceutical and chemical industries, biochemistry and forensic science.
More about capillary electrophoresis Share

CAPEL-105M is a capillary electrophoresis system for fast and effective solutions for food industry, environmental applications, pharmaceutical and chemical industries, biochemistry and forensic science.

principle of operation

High performance capillary electrophoresis is based on the differential migration of components of aqueous samples within a narrow fused silica capillary driven by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high sensitive optical system based on direct or indirect UV absorbance.

features and benefits
Extended instrumental options
  • Spectra scanning facilitates peak identification
  • Broad range of controlled injection pressure allows analysis of viscous samples
  • Reverse sample injection under vacuum: ultra-short analysis time (less than 1 min) and sample stacking to decrease detection limit
Precise and highly effective capillary thermostating provided by circulating liquid coolant (water)(± 0.1 C)
  • Extended range of applied buffers and increased efficiency in separation
Powerful software package "Elforun®"
  • Increased flexibility in performing analyses of various complexity
  • Any kind of complex runs are possible including those with pre-programming of changes in analysis conditions
  • Customized report, data export to other programs
Unique design of the capillary cassettes
  • Easy capillary change in just a few minutes
  • Lower Detection Limit due to the optimized optical scheme
Analytical characteristics

Detectors
CAPEL-105M is equipped with a spectrophotometric detector  with a wavelength range of 190 - 400 nm.

Light source
Deuterium lamp

Vials
Standard Eppendorf-type 1.5 ml, minimal sample volume 100 µl.

Injection modes
Sample injections by pressure, vacuum or electrokinetically.
Controlled pressure profile of injection with pressure values from – 99 mbar to + 99 mbar.
Electrokinetic injection at (+/-) 1 – 25 kV considering time for voltage ramping.
Programmable injection time / pressure / voltage.

Analysis
With a Voltage from -25 kV to +25 kV with a 1 kV step and an electronic polarity switch.
Current from 0 to 200 mA.

Elforun® software features

  • Combination of instrument controlling, data acquisition and data processing;
  • Real time electropherogram visualization;
  • Electropherogram data processing with overlay mode and basic mathematical operations;
  • Computation of electrophoresis system parameters;
  • Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors;
  • Extensive data processing algorithm customization;
  • GLP and GALP compliance.
equipment and options
  • Capillary electrophoresis system CAPEL-105M;
  • Spare capillary cassettes;
  • Elforun® software package;
  • Kits for analyses (by request); most of CE-kits of any manufacturers are compatible with Capel-105M.

Warranty

All Lumex instruments and equipment are covered by a 12-months warranty.

services

Installation of LUMEX instruments can be carried out at a Customer's site by our service engineers. Personnel training specific to the Customer needs can be also provided.

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Detector Spectrophotometer
190-400 nm
Analysis Constant voltage (+/-) 1 - 25 kV in 1 kV steps
Automatic (electronic) polarity switch
Current 0 - 200 mkA
Pressure (+/-) 1-99 mbar
Programmable changing of wavelength, pressure and voltage during analysis
Injection By voltage
By pressure
By vacuum
Sampler Autosamplers for 10 inlet and 10 outlet vials
Rinsing With pressure ~1000 or 2000 mbar
Capillary Length: 30-100 cm
Internal diameter: 50, 75, 100 μm
Capillary thermostating Liquid thermostating conducted at temperatures from 10 ºC below to 30ºC above ambient temperature (± 0.1 C)
Controlled parameters Wavelength
Pressure
Temperature
Voltage
Power requirements 110/220 Vac, 50/60 Hz
Power consumption 200 W
Size, cm 420х570х360
Weight, kg 25
areas of application
  • Environmental analysis: water and soil quality monitoring (cations, anions, herbicides, pesticides);
  • Food industry: analysis of beverages and juices (cations, anions, vitamins, organic acids, preservatives, antioxidants, dyes, sweeteners); mineral water (cations, anions); wines and brandies (cations, organic acids, polyphenols, aldehydes, sulfite); beer (inorganic cations and anions, hop and bitter acids, sulfite, amino acids, organic acids, vitamins); milk (proteins, antibiotics); tea, coffee (caffeine, polyphenols); foodstuff (amino acids, synthetic dyes, organic acids, amines, proteins, melamine);
  • Agriculture: grain and grain products (amino acids), mixed fodders (cations, amino acids, amines, vitamins);
  • Chemical industry: technological monitoring, analysis of raw material;
  • Pharmaceutical industry: patent medicines analysis, technological monitoring, and enantiomers separation;
  • Biochemistry: analysis of proteins by capillary isoelectric focusing technique, pharmacokinetics studies, hemoglobin variants determination;
  • Forensic studies: analysis of explosives and trace detection, drugs analysis, analysis of writing paper components.
Application Product
Determination of preservatives (benzoic, sorbic acids and their salts) and sweeteners (acesulfame K, saccharine and its salts) in food products and raw materials, and dietary supplements Determination of preservatives (benzoic, sorbic acids and their salts) and sweeteners (acesulfame K, saccharine and its salts) in food products and raw materials, and dietary supplements

Capel®-105M

Capel®-205

Determination of water-soluble forms of inorganic cations in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments Determination of water-soluble forms of inorganic cations in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments

Capel®-105M

Capel®-205

Determination of bromide and iodide anions in water samples by Determination of bromide and iodide anions in water samples by "Capel-105M" capillary electrophoresis system

Capel®-105M

Capel®-205

Determination of chlorate, perchlorate, and chlorite ions in drinking water including bottled water Determination of chlorate, perchlorate, and chlorite ions in drinking water including bottled water

Capel®-105M

Capel®-205

Determination of acetic acid (acetate anions) in natural water and wastewater Determination of acetic acid (acetate anions) in natural water and wastewater

Capel®-105M

Capel®-205

Determination of water-soluble forms of inorganic and organic anions in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments Determination of water-soluble forms of inorganic and organic anions in soils, clays, peat, wastewater silt, activated sludge, and bottom sediments

Capel®-105M

Capel®-205

Determination of inorganic anions in aqueous matrices according to EPA 6500 and ASTM D6508-10 test methods Determination of inorganic anions in aqueous matrices according to EPA 6500 and ASTM D6508-10 test methods

Capel®-105M

Capel®-205

Determination of inorganic cations in water samples Determination of inorganic cations in water samples

Capel®-105M

Capel®-205

Determination of inorganic anions in water samples Determination of inorganic anions in water samples

Capel®-105M

Capel®-205

Determination of fructose, glucose, and saccharose in beverages, fruit and vegetable products, honey, and bioactive food additives Determination of fructose, glucose, and saccharose in beverages, fruit and vegetable products, honey, and bioactive food additives

Capel®-105M

Capel®-205

Determination of amino acids in fodders and raw materials using high performance capillary electrophoresys method using Capel-105 Determination of amino acids in fodders and raw materials using high performance capillary electrophoresys method using Capel-105

Capel®-105M

Capel®-205

Determination of tryptophane in fodders and raw materials using high performance capillary electrophoresys method using Capel-105 Determination of tryptophane in fodders and raw materials using high performance capillary electrophoresys method using Capel-105

Capel®-105M

Capel®-205

Determination of serum protein fractions Determination of serum protein fractions

Capel®-105M

Capel®-205

Determination of protein purity and heterogeneity with capillary gel electrophoresis and capillary isoelectric focusing Determination of protein purity and heterogeneity with capillary gel electrophoresis and capillary isoelectric focusing

Capel®-105M

Capel®-205

Determination of caffeine and theobromine in tea, coffee, cocoa, and herbal products Determination of caffeine and theobromine in tea, coffee, cocoa, and herbal products

Capel®-105M

Capel®-205

Identification and determination of synthetic dyes in soft and alcoholic drinks using Capel-105M Identification and determination of synthetic dyes in soft and alcoholic drinks using Capel-105M

Capel®-105M

Capel®-205

Determination of vanillin, sinapic, coniferyl, and syringic aldehydes in brandies and brandy spirits Determination of vanillin, sinapic, coniferyl, and syringic aldehydes in brandies and brandy spirits

Capel®-105M

Capel®-205

Determination of proteins concentrations and their molecular weights using Capel-105M Determination of proteins concentrations and their molecular weights using Capel-105M

Capel®-105M

Capel®-205

Determination of bitter iso- alpha-acids and alpha- and beta-hop acids in beer using Capel-105 systems Determination of bitter iso- alpha-acids and alpha- and beta-hop acids in beer using Capel-105 systems

Capel®-105M

Capel®-205

Determnation of albumin concentration in urine Determnation of albumin concentration in urine

Capel®-105M

Capel®-205

Determination of phenoxycarboxylic acid herbicides in water using high performance capillary electrophoresys method using Capel systems Determination of phenoxycarboxylic acid herbicides in water using high performance capillary electrophoresys method using Capel systems

Capel®-105M

Capel®-205

Determination of caffeine, ascorbic acid, preservatives (benzoic acid, sorbic acid and their salts), and artificial sweeteners (acesulfame K, saccharine) in soft and strong drinks Determination of caffeine, ascorbic acid, preservatives (benzoic acid, sorbic acid and their salts), and artificial sweeteners (acesulfame K, saccharine) in soft and strong drinks

Capel®-105M

Capel®-205

Determination of organic acids in beverages Determination of organic acids in beverages

Capel®-105M

Capel®-205

Determination of inorganic cations in drinks Determination of inorganic cations in drinks

Capel®-105M

Capel®-205

Determination of D- and L-isomers of tartaric and malic acids in wines, wine materials, and food additives Determination of D- and L-isomers of tartaric and malic acids in wines, wine materials, and food additives

Capel®-105M

Capel®-205

Determination of organic acids in feedstuff, silage, haylage, and fermentates for biogas plants Determination of organic acids in feedstuff, silage, haylage, and fermentates for biogas plants

Capel®-105M

Capel®-205

Determination of organic acids and some preservatives in wine and spirits Determination of organic acids and some preservatives in wine and spirits

Capel®-105M

Capel®-205

Determination of total sulfur dioxide in wine and beer Determination of total sulfur dioxide in wine and beer

Capel®-105M

Capel®-205

Determination of hesperidin and naringin in juices using CAPEL-105M capillary electrophoresis system Determination of hesperidin and naringin in juices using CAPEL-105M capillary electrophoresis system

Capel®-105M

Capel®-205

Determination of the carbendazim content in orange and other citrus juice produce Determination of the carbendazim content in orange and other citrus juice produce

Capel®-105M

Capel®-205

Determination of whey proteins in milk samples by capillary gel electrophoresis Determination of whey proteins in milk samples by capillary gel electrophoresis

Capel®-105M

Capel®-205

Determination of amino acids in beer and wort by capillary electrophoresis Determination of amino acids in beer and wort by capillary electrophoresis

Capel®-105M

Capel®-205