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21.12.2018 | Live webinar: New perspectives in performing long sequences of capillary electrophoresis analyses using streaming potential control

 

Date: February 6, 2019 | Time: 11 am PST (7 pm UTC) | Duration: 1 hour

New perspectives in performing long sequences of capillary electrophoresis analyses using streaming potential control

Proper conditioning of the inner capillary surface in capillary electrophoresis (CE) is of utmost importance for the run-to-run reproducibility of the migration times and peak areas of the analytes. Typically, this is accomplished by the capillary rinse.

To assess the quality of the rinse we propose to measure a streaming potential (SP), a phenomenon which arises between the ends of a fused silica capillary when an electrolyte is pressed through it. After a new capillary is thoroughly rinsed, a so-called exemplary SP value is saved in a capillary certificate. Later during the sequence of analyses, SP is automatically measured every time after rinsing and is compared with the exemplary value. A corresponding hardware and software were implemented in the Lumex Instruments Capel-205 CE system.

The SP control can be used to provide long sequences of CE analyses overnight in a fully automated way and to assess the integrity and stability of any kinds of capillary coatings. Moreover, being poorly investigated this topic has great potentials in getting more knowledge both in fundamental aspects and in routine applications, thus setting the stage for new perspective grants and scientific projects.

Register for our upcoming webinar for free to know all about practical use and advantages of the streaming potential control.

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CE system Capel-205 >>