Determination of albumin concentration in urine

INTRODUCTION

The capillary electrophoresis method provides det ermination of albumin concentration in urine.

MEASURING METHOD

Determination of albumin is based on electrophoretic migration of its anionic form in the electric field and its direct detection by measuring the UV absorption in the 215–220-nm range. Prior to analysis sample aliquot was desalted either by ultra-diafiltration or on the “Sephadex® G-25” column.