Determination of serum protein fractions

INTRODUCTION

The method allows determination of relative amou nts of albumin and globulins in human serum using capillary electrophoresis methods.

MEASURING METHOD

Determination of albumin and globulins of the human serum is based on their migration and separation in the electric field due to different electrophoretic mobility. Identification and quantit ative determination of the analysed proteins is performed by direct detection by measuring the UV absorption at 215 nm wavelength.