Determination of methionine hydroxy analogue in fodder additives

INTRODUCTION

The method is used for the determination of the mass fraction of methionine hydroxy analogue (DL-2-hydroxy-4-(methylthio) butyric acid (HMTBa) and its calcium salt) in fodder additives by capillary electrophoresis.

Currently, fodder additives containing HMTBa or its calcium salt, are presented on the market under such brand names as MHA®, Alimet®, Rhodimet®, and others.

For the determination of free methionine in fodder additives use the method М 04-63-2016 (Lumex Instruments set, order No 0300002780).

MEASURING METHOD

The measurement method is based on capillary zone electrophoresis with direct UV detection at the wavelength of 200 nm.