Determination of choline (vitamin B4) in feedstuffs, compound feeds, fodder additives, and feed raw materials

INTRODUCTION

The method is used for the determination of the mass fraction of free choline (synthetic and natural) in feedstuffs by capillary electrophoresis. The method can be applied for all types of feedstuffs, compound feeds, fodders, premixes, fodder additives, and feed raw materials. The present method does not allow the determination of bound choline.

MEASURING METHOD

The measurement method is based on extraction of free choline (vitamin B4) from a sample by water and its determination by capillary electrophoresis with indirect UV detection at the wavelength of 267 nm.