INTRODUCTION

Lumex Instruments proposed the method for determination of the mass fraction of free forms of glutamic acid and its salts (E620–E625) in samples of food products, food raw materials, and food additives by capillary electrophoresis (CE).

Food products can contain substantial amounts of naturally occurring glutamic acid in both free and bound forms. This method determines the total content of free forms of glutamic acid, whether naturally present or added as a food additive. The method does not cover the determination of bound forms of glutamic acid found in proteins and peptides.

MEASURING METHOD

The measurement method is based on extraction of glutamic acid from a solid sample by water and their determination by capillary electrophoresis with direct UV detection at the wavelength of 200 nm.