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Analysis of recombinant protein purity and heterogeneity by capillary electrophoresis

Determination of tryptophane in fodders and raw materials using high performance capillary electrophoresys method using Capel-105

INTRODUCTION

The method enables fast quantitative determination of amino acid tryptophane in feeds, mixed fodders, and raw materials.

MEASURING METHOD

Capillary electrophoresis for the determination of tryptophane is based on differential migration of its ionic form in a quartz capillary under the influence of the applied electric field. Identification of tryptophane is made by measuring its own absorbance at 219 nm wavelength in a borate buffer at 40 °C. Basic hydrolysis of fodder sample is done according to a certified protocol in closed containers with 1.5 M barium hydroxide for 16 hours at 110 °C. After removal of the excess of the base the treated solution is analyzed by capillary electrophoresis.

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Determination of tryptophane in fodders and raw materials using high performance capillary electrophoresys method using Capel-105Download application file
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