Lumex Instruments - Laboratory Analytical Equipment manufacturer Logo
Unit 1-3 7294 Fraserview Place, Mission, B.C., V4S 0A3, Canada V4S 0A3 Unit 1-3 7294 Fraserview Place, Mission, B.C., V4S 0A3 Canada
+1 604-820-8085, +1 604 820-4486, sales@lumexinstruments.com

Lumex Instruments

Insight into mysteries of Nature
  • COVID-19 Detection System
  • Analytical equipment
  • Analytical equipment
  • Analytical equipment
  • Analytical equipment

Products

Lumex Instruments YouTube channel

Determination of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements

Analysis of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements Lumex Instruments 187

INTRODUCTION

The method is used for the determination of the mass fraction of caffeine and theobromine in tea and tea products, coffee and coffee products, cocoa and cocoa products, and dietary supplements.

MEASURING METHOD

Capillary electrophoresis method for the determination of the mass fraction of caffeine and theobromine is based on micellar electrokinetic chromatography (MEKC). Identification and quantitative determination of the components is performed by direct UV detection at the wavelength of 254 nm.

MEASUREMENT RANGE

The measurement range for the components is 100 to 100 000 mg/kg (ppm).
Preservatives (sorbic and benzoic acids), sweeteners (acesulfame K, saccharin, aspartame, cyclamate), sodium glutamate, synthetic dyes, vitamins of the B group, vitamin C, vanillin in the concentrations that are typical for the products of interest does not influence the determination of the components.

File
Determination of caffeine and theobromine in tea, coffee, cocoa, and dietary supplementsDownload application file
EQUIPMENT FOR ANALYSIS
Determination (analysis) of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements Determination of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements - by
5/ 5stars
Analysis of caffeine and theobromine in tea, coffee, cocoa, and dietary supplements