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Lumex Instruments (0890278 B.C. Ltd.) Unit 207, 31510 Gill Ave., Mission, B.C., V4S0A1, Canada 31510 (0890278 B.C. Ltd.) Unit 207, 31510 Gill Ave., Mission, B.C., V4S0A1, Canada
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Analysis of recombinant protein purity and heterogeneity by capillary electrophoresis

Determination of caffeine and theobromine in tea, coffee, cocoa, and herbal products

INTRODUCTION

The determination of caffeine and theobromine content in tea, coffee, cocoa, foodstuffs and in herbal products ensures safety and quality control of the products and identific ation of adulterants.

MEASURING METHOD

The method is based on extraction of the components to be determined from the sample into hot distilled water, their separation and quantitative determination by capillary electrophoresis using the micellar electrokinetic chromatography technique. The components are detected in the UV spectral range at a wavelength of 254 nm.

MEASUREMENT RANGE

The measurement ranges of caffeine and theobromine are 0.1–100 g/kg (ppm) for a sample of 1 g weight. The determination of the components is not hindered by the presence of other food ingredients/additives (sweeteners, preservatives, dyes, vitamins, sodium glutamate, vanillin) if their concentrations are characteristic of the analyzed product.

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Determination of caffeine and theobromine in tea, coffee, cocoa, and herbal productsDownload application file
EQUIPMENT FOR ANALYSIS