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Analysis of recombinant protein purity and heterogeneity by capillary electrophoresis

Determination of amino acids in fodders and raw materials using high performance capillary electrophoresys method using Capel-105

INTRODUCTION

The method enables fast quantitative determination in feeds, mixed fodders, and raw materials of the following amino acids: arginine, lysine, tyro sine, phenylalanine, histidine, leucine and iso -leucine (total), methionine, valine, proline, alanine, glycine, cystine, tryptophan, aspartic and glutamic acids.

Since during the sample decomposition asparagine and glutamine are hydrolyzed to aspartic and glutamic acids, respectively, the content of these two acids represents the total content of both the acids and the amides.

MEASURING METHOD

The determination of amino acids in the samples is made either after preliminary alkaline hydrolysis for tryptophan or after acidic hydrolysis for all the ot her amino acids. Free amino acids are transformed to phenylthiocarbamyl derivatives (PTC derivatives) by m eans of phenylisothiocyanate and their ionic forms are separated in the quartz capillary under the action of an el ectric field. The PTC derivatives are determined by measuring their own absorbance at 254 nm wavelength in a buffer solution.

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Determination of amino acids in fodders and raw materials using high performance capillary electrophoresys method using Capel-105Download application file
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